Dear Sir,
This is Thirunavukkarasu am working in Ordain Health Care
AR&D, Am Having one problem While doing method development
my question Metadoxine and Pyridoxine same chemical nature
and structural activity i tried lot of methods but Analyte
eluted same Retantion time i cant separate both peaks.
solubility, pH both are same only only difference melting
point metadoxine 210 C Pyridoxine 102 C please do the help
needful for me
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Answer Posted / swathi
Once u can use hilic silica column ..mostly water %dont exceed 20% in gradient program otherwise phenyl column particle size 3micron..slow gradient ...flow rate decrease..and at which they are eluting there keep isocratic for 30 min
Is it the compounds are different?
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