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Why do some cell lines require sodium pyruvate?
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Pyruvate is an intermediary organic acid metabolite in
glycolysis and the first of the Embden Myerhoff pathway
that can pass readily into or out of the cell. Thus, its
addition to tissue culture medium provides both an energy
source and a carbon skeleton for anabolic processes. Its
addition may help in maintaining certain specialized cells,
may help when cloning and may be necessary when the serum
concentration is reduced in the medium (Culture of Animal
Cells
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Micro Biology 
