Name an a marker gene other than kanamycin resistance gene
used in plant tissue culture and why it is used now a days?
- 1 Answers
- 3521 Views
- I also Faced
- E-Mail Answers
Answer / ambalika
A new selectable marker system was adapted for use in Agrobacterium-mediated transformation of rice (Oryza sativa L.). This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transformed cells were capable of utilizing mannose as a carbon source. Transgenic rice plants regenerated from selected transformed immature embryo-derived calli on media containing various concentrations of mannose. The highest transformation frequency of 6.0% was obtained when a combination of 25 g/l mannose and 5 g/l sucrose was used. Molecular and genetic analyses showed that the plants contained the pmi gene and the gene was transmitted to the progeny in Mendelian fashion. The results indicated that the mannose selection system, which was devoid of the disadvantages of antibiotic or herbicide selection, could be used for rice Agrobacterium-mediated immature embryo transformation.
| Is This Answer Correct ? | 0 Yes | 0 No |
What is necessary for commercial production of a biotech plant?
Name the dye commonly used as the counterstain in the Gram staining process.
can u give information regarding the institutes offering project training for m.sc completed students and further allocating job oppurtunities
what is the process of chees preparation
if i do b.sc elective biotechnolgy and after that m.sc , how much sallary i will get in this field ??????
Suppose that an investigator wanted to use DNA fingerprinting to associate a blood sample with a criminal. What materials would be necessary?
what is the difference between transfection and transduction?why the word trasfection is prefers for transfer of genetic material through non viral particles in eukaryote not for prokaryote??
Explain Gram Staining method?
i have passed my +2 i am getting admission biotechnolgy in govt college chd afflited to punjab universty, but beside that i am getting admission in b.pharma in punjabi universty patiala where should i go in which field
What are some techniques that are used to transfer DNA in plants?
hi! i hv done my bachelors in microbiology n masters in biotechnology.....pls tell me wat wud b the nxt option yaar...it wud b helpful
What kinds of metrics would you gauge to determine the financial, strategic and operational health of a prospective alliance partner?
Micro Biology 
