what laboratory procedures are followed when working with microorganisms?

what laboratory procedures are followed when working with
microorganisms?

Question Posted / zikku

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what laboratory procedures are followed when working with microorganisms?..

Answer / chetali

1. To minimise the risk of spreading microbial
contamination, you should have a laboratory coat that you
use only for microbiology work.
2. All cuts or abrasions should be protected with
waterproof plasters before starting work. Hands must be
disinfected or washed immediately when contamination is
suspected, after handling infective materials and also
before leaving the laboratory (even if only leaving for a
short time) using soap and warm water at the wash-hand
basins provided.
3. The laboratory door must be closed when work is in
progress. Bench surfaces must be disinfected beforehand.
4. No work should commence unless adequate discard
facilities, appropriate to the work being undertaken, are
available.
5. Use all sterilized glassware, equipment and medias to
avoid any contamination.
6. Sterilise all the waste cultural medias in an autoclave
before disposal.
7. Label each and every item.
8. Tidy up after use, do not leave any unidentified
cultures, solutions etc. on the bench, and decontaminate
the work surface with a disinfectant-soaked mop followed by
drying with paper towel before leaving the laboratory.

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what laboratory procedures are followed when working with microorganisms?..

Answer / jenna

It really depends on the organism. If you're working with
a mycobacterium or dangerous fungi then most work is
performed under a ventilated hood. The microbiologist will
wear a disposable respirator, gloves, and a gown (or PPE -
Personal Protective Equipment). I'm a student in a fairly
large microbiology lab and for the most part people just
clean their benches at the beginning of every shift and
wash their hands periodically throughout. Most people here
unless they're working directly with a specimen don't even
wear gloves, but everyone wears their PPE. Pants and close-
toed close-heeled shoes are mandatory. It's a good idea to
cover up any cuts you have. When in doubt just keep gloves
on at all times and wash your hands each time you change
them.

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