Which form of plasmid DNA moves quickest thro Agarose gels?

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Question Posted / maha thilak

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Which form of plasmid DNA moves quickest thro Agarose gels?..

Answer / ramesh singh kunjwal

Plasmid is present in the bacterial cell in the three
distinct forms.
1. Supercoiled
2. Nicked
3. Linear
Through the agarose gel the Supercoiled form move
Quickest.

Is This Answer Correct ?

40 Yes

4 No

Which form of plasmid DNA moves quickest thro Agarose gels?..

Answer / saurabh gayali

Circular DNA rather than linear

Is This Answer Correct ?

27 Yes

12 No

Which form of plasmid DNA moves quickest thro Agarose gels?..

Answer / ambalika

In order, from slowest running to fastest running, they are relaxed or nicked circular (supercoling not yet accomplished [relaxed] or removed [nicked, a single stranded break]), linear (double stranded break), and supercoiled.

Is This Answer Correct ?

10 Yes

2 No

Which form of plasmid DNA moves quickest thro Agarose gels?..

Answer / sourav saha

There are 3 conformation observed on 1% agarose gel run of isolated Plasmid DNA, they are -- supercoiled circular DNA, nicked supercoiled circular DNA, and relaxed circular Dna. If the isolated plasmid DNA is not treated with Rnase enzyme then another band is observed in agarose gel known to be DNA masked with RNA. Read lehninger and stryer.

Is This Answer Correct ?

6 Yes

2 No

Which form of plasmid DNA moves quickest thro Agarose gels?..

Answer / sridhar

usually on the basis of mol weight the speed of DNA
depends..so the answer is single std DNA

Is This Answer Correct ?

7 Yes

10 No

Which form of plasmid DNA moves quickest thro Agarose gels?..

Answer / subhash

I think its the linearized form.

Is This Answer Correct ?

11 Yes

30 No

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