Which form of plasmid DNA moves quickest thro Agarose gels?
Answers were Sorted based on User's Feedback
Answer / ramesh singh kunjwal
Plasmid is present in the bacterial cell in the three
Through the agarose gel the Supercoiled form move
|Is This Answer Correct ?||40 Yes||4 No|
Answer / saurabh gayali
Circular DNA rather than linear
|Is This Answer Correct ?||27 Yes||12 No|
Answer / ambalika
In order, from slowest running to fastest running, they are relaxed or nicked circular (supercoling not yet accomplished [relaxed] or removed [nicked, a single stranded break]), linear (double stranded break), and supercoiled.
|Is This Answer Correct ?||10 Yes||2 No|
Answer / sourav saha
There are 3 conformation observed on 1% agarose gel run of isolated Plasmid DNA, they are -- supercoiled circular DNA, nicked supercoiled circular DNA, and relaxed circular Dna. If the isolated plasmid DNA is not treated with Rnase enzyme then another band is observed in agarose gel known to be DNA masked with RNA. Read lehninger and stryer.
|Is This Answer Correct ?||6 Yes||2 No|
Answer / sridhar
usually on the basis of mol weight the speed of DNA
depends..so the answer is single std DNA
|Is This Answer Correct ?||7 Yes||10 No|
Answer / subhash
I think its the linearized form.
|Is This Answer Correct ?||11 Yes||30 No|
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