what are lac operons?

5 Answers   Biological.e.limited,

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Name a sulphur containing amino acid.

0 Answers  

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what are the genetic defects in urea cycle?

1 Answers  

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what is lactam-lactim tautomerism?

3 Answers   Reddy Labs,

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Since pepsin is a gastric enzyme does it, has acid or basic optimum ph? What happen to pepsin when it passes to the duodenum?

0 Answers  

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how rotenone acts as an inhibitor of electron transport?

1 Answers   Ion Exchange,

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what r artificial sweeteners?

1 Answers  

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What is the main enzyme that participates in dna duplication process?

0 Answers  

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What are the favorable conditions for formation of Anions?

0 Answers  

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what are the essential aminoacids?

4 Answers   Nutrition Research Laboratories,

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

0 Answers  

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Compare endonuclease and exonuclease?

5 Answers   Biocon,

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