According to the fluid mosaic model of cell membranes, which type of molecule spans the membrane from its inner to outer surface? a) cholesterol b) phospholipid c) protein d) triacylglycerol

1 Answers   PH.D Interview,

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

0 Answers  

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What is hapten?

3 Answers   Reddy Labs,

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Covalent bond is directional therefore which covalent property is shown?

0 Answers  

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how bpg acts as a haemoglobin regulator?

2 Answers  

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Explain the reduction of the activation energy of chemical reactions?

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what is meant by panhypopituitarism?

1 Answers  

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The ATP synthase of bacteria is an Fl-like particle attached to the a) ends of proto-mitochondria. b) inner surface of the cell (or plasma) membrane. c) outer surface of the cell (or plasma) membrane. d) surface of protonic vesicles.

1 Answers  

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Define activation energy?

0 Answers  

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What is the difference between pH and pKa?

3 Answers   Astra,

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How is the cooling of organs and tissues for medical transplantations associated with the effect of temperature upon enzymatic reactions?

0 Answers  

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Define geometrical isomerism.

0 Answers  

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