What does the Iodine mordant do in a gram stain?

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Question Posted / sameer singh faujdar

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What does the Iodine mordant do in a gram stain?..

Answer / sameer singh faujdar

By definition a mordant is a substance that set dyes. It doesn't really contribute to the colouring of the bacteria. In gram staining procedure iodine act as mordant by making crystal violet-iodine complex which actually retains in gram positive bacteria and not in gram negative bacteria, because of this complex gram positive bacteria appears violet in colour.

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What does the Iodine mordant do in a gram stain?..

Answer / biotrick.com

Iodine is used to fix the dye crystal violet

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What does the Iodine mordant do in a gram stain?..

Answer / laxmi kumari

it act as a mordant to stick crystal violet to the cell .

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What does the Iodine mordant do in a gram stain?..

Answer / bhupathi.e

Iodin enhances reaction between bacterial cell wall and crystal violet dye for giving violet colour

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What does the Iodine mordant do in a gram stain?..

Answer / laxmi

it removes extra stain from slide

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