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Describe Southern Blotting technique?
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Answer / mcrb
1. isolate DNA content of cell, and perform the digestion
with restriction enzyme.
2. separate by gel electrophoresis and perform the alcali
treatment
3. put nitrocellulose or nylon membrane on gel. ends of
filter paper in buffer.
4. put towels of filter parer and put weight on top.
5. solution moves up due to capillary action along with DNA
on gel slab.
6. dry filter paper.
7. let hybridization with radio labelled or fluorescent
probes.
8. wash out unhybridised probes.
9. detect hybridisation.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / madhuraka
1. isolate protein content of cell.
2. separate by gel electrophoresis.
3. put nitrocellulose or nylon membrane on gel. ends of
filter paper in buffer.
4. put towels of filter parer and put weight on top.
5. solution moves up due to capillary action along with DNA
on gel slab.
6. dry filter paper.
7. let hybridization with radio labelled or fluorescent
probes.
8. wash out unhybridised probes.
9. detect hybridisation.
| Is This Answer Correct ? | 1 Yes | 2 No |
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