Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

Describe Southern Blotting technique?

Answer Posted / madhuraka

1. isolate protein content of cell.
2. separate by gel electrophoresis.
3. put nitrocellulose or nylon membrane on gel. ends of
filter paper in buffer.
4. put towels of filter parer and put weight on top.
5. solution moves up due to capillary action along with DNA
on gel slab.
6. dry filter paper.
7. let hybridization with radio labelled or fluorescent
probes.
8. wash out unhybridised probes.
9. detect hybridisation.

Is This Answer Correct ?    1 Yes 2 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is the relation between concentration gradient and active and passive transport?

938


What are the two main types of endocytosis?

967


Give an account of frozen plant tissue organelle isolation ?

1665


What is cellulose?

878


How to properly prepare glycyrrhizic acid solution ?

1864


What is meant by suction force of the plant cell? Does the suction force facilitate or make difficult the entrance of water in the cell?

945


what is Baculovirus Expression ?

2564


Define cell biology?

852


Are PCR primers used for Mycoplasma detection?

1751


What is autophagic intracellular digestion? Why is this type of intracellular digestion intensified in an organism undergoing starvation?

972


what is the role of L-glutamine in RPMI?

2203


What is spherical cell like contamination ?

1808


What is the relation between the concepts of chromatin and chromosome? Are euchromatin and heterochromatin part of chromosomes?

877


what are connecting proteins ?

1851


what is the proper name for bottle holder thingee ?

1971