Interested to Buy Any Domain ? << Click Here >> for more details...
How to use agar for antibiotic sensitivity testing ?
- 2 Answers
- 5949 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / yasodha
Muller hinton agar is added to sterile water and
autoclaved.Thi is poured in to petri dishes to get 2 mm of
thickness under sterile conditions,These plates are stored
in refrigirater for storage.For sensitivity,make lawn
culture on media and keep antibiotic discs in radial
fashion at equal distance(not more than 6).Incubate at 37
degree centigrades for 24 hrs.So that sentivity zones
appear around the discs.Read the zone size with scale for
sensitivity.
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / shweta
For antibiotic sensitivity testing prepare a known
concerntration of agar medium by desolving it in sterile
d/w .After getting a transperent solution pour it in petri
dishes by maintaining a thikness of 2mm and let it set
down .Keep it in hot air oven for 1 min for removal of
moisture so that it does not creat any contamination. Take
the plates and place different antibiotics my maintaining
distance of 24 from each other. So in that way agar is used
for antibiotic sensitivity testing.
| Is This Answer Correct ? | 1 Yes | 3 No |
Explain the difference between nematocyst and spicules?
who is the father of genatics
what are the applications of ELISA assays and what is the procedure of ELISA test?
What do all organic compounds contain?
Order the following from the part of the digestive tract nearest to the beginning to the part nearest to the end: jejunum, ileum, esophagus, duodenum?
What is the highest temperatures humans can survive?
What is a brain freeze?
How do environmental factors affect growth ?
Why do leaves change color?
What is the difference between food infection and food?
How to do Petrifilm?
Explain why do organisms live in certain places?
Micro Biology 
