How to culture virus in primary cell lines?
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Answer / Harsh Kumar
Culturing viruses in primary cell lines involves infecting the cells with the virus and maintaining them under controlled conditions. Here are some general steps: (1) preparation of primary cells, which can be obtained from various tissues or organs, (2) seeding the cells into culture vessels, (3) inoculating the cells with the virus, (4) incubating the cells at an appropriate temperature and atmosphere, (5) observing the cells for cytopathic effects (CPE), which are changes in the cell morphology caused by the virus, and (6) harvesting the virus from the culture medium.
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