what is Sauton's media recipe?
- 2 Answers
- 14739 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Ingredients are
KH2PO4 0.5 g
MgSO4.7H2O 0.5 g
Citric acid 2.0 g
Ferric ammonium citrate 0.05 g
Glycerol 60 ml
Asparagine 4.0 g
Distilled water to 1000 ml.
Dissolve the reagents, except for the glycerol, in 300 ml
water using heat.
Once dissolved, add the glycerol and make up to 1 liter
with distilled water. Check pH and, if necessary, adjust to
pH7.4 with 1 M NaOH.
Autoclave at 121 oC for 15 min.
It has been reported that the addition of ZnSO4 (0.1 ml of
1% solution) and Triton WR1339 (5 ml of 5% solution)
improves growth of Mycobacterium. tuberculosis .
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / b.yazdanpanah
Ingredients are
KH2PO4 0.5 g
MgSO4.7H2O 0.5 g
Citric acid 2.0 g
Ferric ammonium citrate 0.05 g
Glycerol 60 ml
Asparagine 4.0 g
Distilled water to 1000 ml.
Dissolve asparagin separatly in 100 ml distilled water
using magnet stirer & heat upto 56 Deg C
After dissolve the other reagents, except for the glycerol,
in 300 ml water one by one,using magnet stirer , add the
asparagin solution and after 5 minuts add glycerol and then
make up to 1 liter.
with distilled water. Check pH and, if necessary, adjust to
pH =7.2 with 1 M NaOH or NH3 solution.
Autoclave at 121 oC for 15 min.
It has been reported that the addition of ZnSO4 (0.1 ml of
1% solution) improve growth of Mycobacteria.
| Is This Answer Correct ? | 0 Yes | 1 No |
Root surface caries is caused by which microorganisms?
What is the amoeba?
What are the Two rapid tests for diagnosis of cholera?
Differentiate between doubling time and generation time?
What are the tiny molecules of naked circular RNA that infect plants called?
Why should the slide be flooded with strong carbol fuchsin?
Expand RAPD ?
What are X & V factors?
why OD measurement for bacteria should be under 60 ?
What is Cholera red reaction ?
What are Beta-lactams?
what are the symptoms of lime disease?
Micro Biology 
