Answer Posted / b.yazdanpanah
Ingredients are
KH2PO4 0.5 g
MgSO4.7H2O 0.5 g
Citric acid 2.0 g
Ferric ammonium citrate 0.05 g
Glycerol 60 ml
Asparagine 4.0 g
Distilled water to 1000 ml.
Dissolve asparagin separatly in 100 ml distilled water
using magnet stirer & heat upto 56 Deg C
After dissolve the other reagents, except for the glycerol,
in 300 ml water one by one,using magnet stirer , add the
asparagin solution and after 5 minuts add glycerol and then
make up to 1 liter.
with distilled water. Check pH and, if necessary, adjust to
pH =7.2 with 1 M NaOH or NH3 solution.
Autoclave at 121 oC for 15 min.
It has been reported that the addition of ZnSO4 (0.1 ml of
1% solution) improve growth of Mycobacteria.
Is This Answer Correct ? | 0 Yes | 1 No |
Post New Answer View All Answers
Which are the theories of gram staining?
Explain the Thawing of competent bacterial cells ?
What are the major uses of hexachlorophene, chlorhexidine, and triclosan as agents for controlling microorganisms?
What is the Transposon for STM ?
Tell something about Blue colored fungi?
what are the microbes that grow if proper temperatures are not maintained during fermentation process?
What is the use of FAM and other green flourescent dyes ?
Give an account of Adenovirus ?
Explain the importance of species of the molds Ustilago and Claviceps in medicine.
Why does frame shift mutation (GC gase paired) occurs due to the pesticides in TA98 salmonella tester strain? What is its reason and the mode of action of the pesticides on the CG sequence of the DNA of TA98 strain
Can you identify the operational bases and uses for the transmission and scanning electron microscopy?
What are the adaptations of Halophites to salinity ?
mention few medical representative companies around mysore
What are the articles sterilized in a hot air oven?
In 1884, the danish microbiologist hans christian gram invented the gram stain, which delineates the difference between what two general types of bacteria?