what are satellite colonies?

Question Posted / guest

3 Answers
65950 Views
I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

what are satellite colonies?..

Answer / kami

Satellite colonies are tiny colonies growing around an
antibiotic resistant colony. The antibiotic resistant colony
releases enzymes that degrade the antibiotic and therefore
benefits the surrounding bacteria.

You can usually tell which colonies are resistant and which
are satellite colonies due to the size.

Is This Answer Correct ?

64 Yes

5 No

what are satellite colonies?..

Answer / shahzad a pandith

On agar plates, ampicillin degradation can lead to the formation of satellite colonies on transformation plates. Satellite colonies are very small colonies of cells that have not taken up the plasmid that form around a large colony that has taken up the bla-containing plasmid. The satellites form because the beta-lactamase released by the bla-expressing colony degrades the ampicillin in the vicinity of the colony. The satellites are not necessarily a problem as they will not grow when transferred to a medium containing fresh ampicillin. Good Luck.

Is This Answer Correct ?

23 Yes

0 No

what are satellite colonies?..

Answer / satendra choudhary

satellite colonies grow when
selecting colonies resistant to Ampicillin, resistance is
mediated by a
beta-lactamase which destroy the antibiotic: so when the
colonies are big
enough, the neighbouring untransformed cells (they are
alive because Ampi
is a bacteriostatic) can form "micro" colonies around the
transformed
cell on a part of medium with less or no antibiotic.

Is This Answer Correct ?

27 Yes

14 No

Post New Answer