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what are the applications of ELISA assays and what is the
procedure of ELISA test?

Answers were Sorted based on User's Feedback



what are the applications of ELISA assays and what is the procedure of ELISA test?..

Answer / ravi

Enzyme-linked immunosorbent assay (ELISA), is a analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.
The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. Attempting to detect (and quantify) the presence of the antigen in the sample proceeds as follows: Antigens from the sample are attached to a surface. Then a further specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.
http://en.wikipedia.org/wiki/ELISA

Is This Answer Correct ?    4 Yes 0 No

what are the applications of ELISA assays and what is the procedure of ELISA test?..

Answer / guest

Elisa is especially used for pregnency detection,it is done
on a slide made for elisa test

Is This Answer Correct ?    0 Yes 5 No

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