what are the applications of ELISA assays and what is the
procedure of ELISA test?
Answer Posted / ravi
Enzyme-linked immunosorbent assay (ELISA), is a analytic biochemistry assay that uses one sub-type of heterogeneous, solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.
The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. Attempting to detect (and quantify) the presence of the antigen in the sample proceeds as follows: Antigens from the sample are attached to a surface. Then a further specific antibody is applied over the surface so that it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.
http://en.wikipedia.org/wiki/ELISA
Is This Answer Correct ? | 4 Yes | 0 No |
Post New Answer View All Answers
What is a graduated cylinder?
How much salt is in the human body?
Hi,I have recieved an i-20 from 2 years college for my bachelors degree,how can i convince the consular about this course i.e.computer and information science.plz help me as soon as possible.
How test tube brush is used?
Define black mold?
In which animals plasma Haemoglobin dissolves
How does oak blight spread?
Explain why is it important to be familiar with the laboratory apparatus and their uses?
Define protein?
What is a distilling flask?
Explain the uses of formycin b?
How is pipette used?
Define triglyceride?
What are some examples of chemical changes?
Explain what is an analogy for microtubules?