how to mesure a relation of assay by HPLC and purity in results?
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Answer / Neha Gautam
In HPLC (High-Performance Liquid Chromatography), the purity and assay of a substance can be measured using the area percentage method. This involves measuring the areas under each peak in the chromatogram and comparing them to a standard. The assay is calculated as the percentage of the active ingredient, while the purity indicates the proportion of the total mixture that consists of the active ingredient.
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