1)how can u determine the concentration in an atp solution.
(iisc phd interview)
2)why snake venum toxin is not acting as toxin to snake
- 2 Answers
- 11144 Views
- IISc, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / vivek
Most of Snake venom contain principle toxin which is PLA2,
which causes lysis of cell membrane. Researcher have found
that in venom it contains PI (PLA2 inhibitor) which inturn
regulates the degradation of own host cell membrane of Snake
itself.
Concentration of ATP can be determine by Luciferase couple
enzyme assay, MS, HPLC or by some other means.....
| Is This Answer Correct ? | 14 Yes | 0 No |
Which cellular compartment becomes acidic during mitochondrial electron transport?
how do the two comonents of starch differ?
What is the structural formula of molybdenum blue?
What is the chemical formula of xylose?
what are the symptoms of the disease phrynoderma?
Is protein biggerrr than RNA
explain the working of monochromator unit?
How to estimate Pneumococcal polysaccharide content by wich methods ? PCV 14 valent
what is the difference between alpha carotene and beta carotene?
Why the transition metal ions or compounds exhibit color?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
which property among the following generally decreases from top to bottom in a group 1a? 1. Electropositive, density, basic nature, classical reactivity
Micro Biology 
