Bio Chemistry Interview Questions
Questions Answers Views Company eMail

Autoanalyser is used for[tick your right choice} 1.Analysing heart defect 2.Imaging various internal body parts 3.Estimating requirement of surgical removal or correction 4.Estimating quantity of various biochemicals in body fluids

4 2226

southern blot technique is related to--------- [fill the gaps]

8 4126

DO POLYSACCHARIDES(STARCH)ANSWER BARFOED'S TEST?IF THEY DO,WHAT IS THE REASON?IF THEY DO NOT,WHAT IS THE REASON?

5 12095




What are advantages of using immolized enzymes?

6 3449

how much the size of nitrocellulose membrane should be in southern blotting.

1 1516

what is the poresize of nitrocellulose membrane in southern blotting.

1 1942

Why hydrolyzed sucrose and starch give positive result with Benedict's reagents?

4 14599

is the situation of hypoglycemia conducive to the secretion of glucagon hormone?why is there a spurt in the secretion of glucagon hormone in adolescence?

3 2055




does glucagon secretion induce sleep in teenagers?why?

962

is there a hormone in our body which when released induces sleep in us?is there a spurt in the secretion of that hormone in adolescence?why?

1 1582

what is ph?

Exel,

6 3391

What is hydrophobic interaction chromatography????

1 2007

name the matrix used in hydrophobic interaction chromatography?????

1 1848

What is Michaelis Menten equation ????

4 3377

why lysine,threonine,proline does not undergo trans amination?

Kuwait University, Dr Reddys,

2 11136


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Un-Answered Questions { Bio Chemistry }

How much empty space is found in globular proteins?

858


I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.

220


When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.

871


What are the structures of the products of this reaction, and how are they identified?

876


Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.

928






How are coupled reactions used in biochemistry?

1091


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

889


Where do hydrophobic and hydrophilic residues usually end up after protein folding?

876


does glucagon secretion induce sleep in teenagers?why?

962


which fat soluble vitamin has co-enzymic function?

757


how I cell disease is caused?

1175


Describe the environment where you do your best work in an organization?

929


Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

889


what are the steps involved in the calibration of HPLC

1412


why we use 96 well plate in ELISA plate reader?

1091