Bio Chemistry Interview Questions
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When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.

889

The reduction-oxidation (Redox) potentials of NADH and FADH2 show that the following reaction is spontaneous: a) FADH2 + NAD+ --> FAD + NADH b) FADH2 + NADP+ --> FAD + NADPH c) NADH + FAD --> NAD+ + FADH2 d) Reactions a) and b) are both spontaneous

1 5653

The ATP synthase of bacteria is an Fl-like particle attached to the a) ends of proto-mitochondria. b) inner surface of the cell (or plasma) membrane. c) outer surface of the cell (or plasma) membrane. d) surface of protonic vesicles.

1 2143




The yield of ATP/glucose unit in glycolysis with glycogen as the glucose source is a) 2.0 b) 2.5 c) 3.0 d) 4.0

1 1920

What was the primary antibody that was used?

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1 3014

Describe how you used that antibody to visualize β-gal protein?

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1 4221

Review your data from the entire module. Say we ask you to redo the purification.Is there any step that you could eliminate? Which one? Why can you eliminate it?

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2 2257

Why do proteins that recognize specific DNA sequences usually bind in the major groove of DNA?

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2 2497




An unusual structure is formed at the 5? end of each cellular mRNA. What is it? After this has formed, does the mRNA have a 5? terminus? Explain.

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1 1986

Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?

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1 2382

Many genes are regulated in both a negative and a positive manner. The genes mediating lactose metabolism in bacteria are a classical example. What are the two small molecule ligands that control expression of these genes? What does each one do?

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1 2074

Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?

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1 2464

Is there a biochemical test for maltose?

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2 15202

When starch reacts with iodine, why does it create the characteristic blue-black colour?

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5 15263

Explain about RESPIRATION ?

1 2081


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Un-Answered Questions { Bio Chemistry }

What type of column is generally used to separate amino acids from each other?

960


Why does physical exertion increase symptoms of poisoning by trematol?

1508


How do peptides react with cyanogen bromide?

792


Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

1963


How are free energy changes related to enthalpy changes and entropy changes?

842






which fat soluble vitamin has co-enzymic function?

777


Which organ and subcellular site are most important for fatty acid biosynthesis?

997


what is meant by codan optimisation m rna enrichment?

1327


What is the structural formula of molybdenum blue?

2276


what is QC and why is impotent in pathology lab

1527


Describe the structure of a peptide giving the sequence.

714


How does a random coil differ from an irregularly structured region?

982


what is your aspiration in profession/ career?

1518


What is zone electrophoresis?

928


DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.

899