Bio Chemistry Interview Questions
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When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.

938

The reduction-oxidation (Redox) potentials of NADH and FADH2 show that the following reaction is spontaneous: a) FADH2 + NAD+ --> FAD + NADH b) FADH2 + NADP+ --> FAD + NADPH c) NADH + FAD --> NAD+ + FADH2 d) Reactions a) and b) are both spontaneous

1 5722

The ATP synthase of bacteria is an Fl-like particle attached to the a) ends of proto-mitochondria. b) inner surface of the cell (or plasma) membrane. c) outer surface of the cell (or plasma) membrane. d) surface of protonic vesicles.

1 2242




The yield of ATP/glucose unit in glycolysis with glycogen as the glucose source is a) 2.0 b) 2.5 c) 3.0 d) 4.0

1 2027

What was the primary antibody that was used?

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1 3076

Describe how you used that antibody to visualize β-gal protein?

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1 4279

Review your data from the entire module. Say we ask you to redo the purification.Is there any step that you could eliminate? Which one? Why can you eliminate it?

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2 2331

Why do proteins that recognize specific DNA sequences usually bind in the major groove of DNA?

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2 2570




An unusual structure is formed at the 5? end of each cellular mRNA. What is it? After this has formed, does the mRNA have a 5? terminus? Explain.

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1 2063

Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?

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1 2438

Many genes are regulated in both a negative and a positive manner. The genes mediating lactose metabolism in bacteria are a classical example. What are the two small molecule ligands that control expression of these genes? What does each one do?

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1 2157

Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?

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1 2540

Is there a biochemical test for maltose?

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2 15385

When starch reacts with iodine, why does it create the characteristic blue-black colour?

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5 15643

Explain about RESPIRATION ?

1 2172


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Un-Answered Questions { Bio Chemistry }

The nucleophile in the first stage of the serine protease mechanism is a) H2O b) the carboxyl of Asp 102 c) the hydroxyl of Ser 195 d) the imidazole of His 57

997


Why does physical exertion increase symptoms of poisoning by trematol?

1576


Which compound is involved in reducing levels of homocysteine in the blood?

1149


why we use 96 well plate in ELISA plate reader?

1153


Why are free cysteines treated with iodoacetate prior to protein sequencing?

1119






Where do hydrophobic and hydrophilic residues usually end up after protein folding?

949


During a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?

1790


I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.

313


How are coupled reactions used in biochemistry?

1175


What is the structural formula of molybdenum blue?

2388


bane the by products when crude oil is refined by distillation

1026


Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

2015


What is zone electrophoresis?

972


i have done M.SC. in biochemistry from india. as i have experience of pharmaceuticals in quality assurance department of 4 year.now i come to know that my degree is valid in uk.i got my comparision letter of degree from uknaric. but i m confuse that what is the next step to do for getiing job in lab? am i eligible to get directly job or as trainee than job ? or to register in ibms or what? pls tell me

2190


What type of column is generally used to separate amino acids from each other?

1008