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Bio Chemistry Interview Questions
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When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.


The reduction-oxidation (Redox) potentials of NADH and FADH2 show that the following reaction is spontaneous: a) FADH2 + NAD+ --> FAD + NADH b) FADH2 + NADP+ --> FAD + NADPH c) NADH + FAD --> NAD+ + FADH2 d) Reactions a) and b) are both spontaneous

1 6664

The ATP synthase of bacteria is an Fl-like particle attached to the a) ends of proto-mitochondria. b) inner surface of the cell (or plasma) membrane. c) outer surface of the cell (or plasma) membrane. d) surface of protonic vesicles.

1 3125

The yield of ATP/glucose unit in glycolysis with glycogen as the glucose source is a) 2.0 b) 2.5 c) 3.0 d) 4.0

1 2890

What was the primary antibody that was used?

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1 3887

Describe how you used that antibody to visualize β-gal protein?

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1 5073

Review your data from the entire module. Say we ask you to redo the purification.Is there any step that you could eliminate? Which one? Why can you eliminate it?

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2 3356

Why do proteins that recognize specific DNA sequences usually bind in the major groove of DNA?

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2 3833

An unusual structure is formed at the 5? end of each cellular mRNA. What is it? After this has formed, does the mRNA have a 5? terminus? Explain.

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1 2850

Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?

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1 3412

Many genes are regulated in both a negative and a positive manner. The genes mediating lactose metabolism in bacteria are a classical example. What are the two small molecule ligands that control expression of these genes? What does each one do?

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1 3217

Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?

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1 3439

Is there a biochemical test for maltose?

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2 17015

When starch reacts with iodine, why does it create the characteristic blue-black colour?

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5 18423

Explain about RESPIRATION ?

1 2947

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Un-Answered Questions { Bio Chemistry }

Define geometrical isomerism.


Define ionic product of water. What is its value?


How much amount of energy is required to break the triple bond in nitrogen molecule?


What are hydrophobicity scales, and how are they used?


Which is element exhibit allotrope in the 6tha group?


Aldehydes (ch3cho) and vinyl alcohol (ch2=choh) are?


How does temperature affect the action of enzymes upon their substrates?


What does ammonal contain? For what purpose it is used?


Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O


What is threshold energy?


What are the main factors that alter the speed of enzymatic reactions?


Why the 2a group elements are called alkaline earth elements?


What factors can change the pKa value of an amino acid residue, and in which direction?


Why ethylene undergoes electrophilic addition reactions?


What is nucleophilic addition reaction?