Bio Chemistry Interview Questions
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When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.


The reduction-oxidation (Redox) potentials of NADH and FADH2 show that the following reaction is spontaneous: a) FADH2 + NAD+ --> FAD + NADH b) FADH2 + NADP+ --> FAD + NADPH c) NADH + FAD --> NAD+ + FADH2 d) Reactions a) and b) are both spontaneous

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The ATP synthase of bacteria is an Fl-like particle attached to the a) ends of proto-mitochondria. b) inner surface of the cell (or plasma) membrane. c) outer surface of the cell (or plasma) membrane. d) surface of protonic vesicles.

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The yield of ATP/glucose unit in glycolysis with glycogen as the glucose source is a) 2.0 b) 2.5 c) 3.0 d) 4.0

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What was the primary antibody that was used?

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1 4262

Describe how you used that antibody to visualize β-gal protein?

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1 5385

Review your data from the entire module. Say we ask you to redo the purification.Is there any step that you could eliminate? Which one? Why can you eliminate it?

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2 3827

Why do proteins that recognize specific DNA sequences usually bind in the major groove of DNA?

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2 4402

An unusual structure is formed at the 5? end of each cellular mRNA. What is it? After this has formed, does the mRNA have a 5? terminus? Explain.

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1 3197

Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?

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1 3810

Many genes are regulated in both a negative and a positive manner. The genes mediating lactose metabolism in bacteria are a classical example. What are the two small molecule ligands that control expression of these genes? What does each one do?

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1 3525

Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?

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1 3747

Is there a biochemical test for maltose?

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2 17480

When starch reacts with iodine, why does it create the characteristic blue-black colour?

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5 19320

Explain about RESPIRATION ?

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Un-Answered Questions { Bio Chemistry }

What are pentoses? To what organic group do pentoses belong?


What is saytzeff’s rule?


What are enzymes? What is the importance of enzymes for the living beings?


Why is not it correct to assert that dna self-replicates?


Which are the nucleotides “portions” that bind in the formation of nucleic acids? What is meant by the 5’ and 3’ extremities of nucleic acids?


What are the main theoretical models that try to explain the formation of the enzyme-substrate complex?


The nucleophile in the first stage of the serine protease mechanism is a) H2O b) the carboxyl of Asp 102 c) the hydroxyl of Ser 195 d) the imidazole of His 57


Name two compounds, which is mixture of lucas reagent?


What is polymerase chain reaction used for?


What is threshold energy?


What are allosteric enzymes?


Why can it be said that the enzymatic action is highly specific?


why life span of RBC IS GREATER THEN THE WBC?


What is Co-ordination number?


Name the first synthetic organic compound. Who proposed it and from which compound?