Bio Chemistry Interview Questions
Questions Answers Views Company eMail

What is a plasmalogen? What do plasmalogens do?

1 5541

What is the role of PAPS?

1 2198

what is Alzheimer's disease?what are its symptoms?

1 3238




Compare and contrast feedback inhibition and enzyme repression?

8107

The peptide bond in proteins is a) nonpolar, and fixed in a trans conformation. b) nonpolar, but rotates to three preferred dihedral angles. c) planar, and usually found in a trans conformation. d) planar, but rotates to three preferred dihedral angles.

1 1771

If the enthalpy change for a reaction is zero, DG? is equal to: a) DH? b) lnKeq c) TDS? d) -TDS?

1 1965

The nucleophile in the first stage of the serine protease mechanism is a) H2O b) the carboxyl of Asp 102 c) the hydroxyl of Ser 195 d) the imidazole of His 57

947

The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

1908




DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.

899

The two exonuclease activities of DNA polymerase I a) are coupled during the formation of "gapped DNA". b) degrade DNA in a 5' --> 3' direction. c) degrade DNA in a 3' --> 5' direction. d) occur at two different active sites.

1 1548

A principal difference between prokaryotic and eukaryotic DNA replication is a) completely different proteins/enzymes in eukaryotes b) multiple origins in eukaryotes c) no requirement for topoisomerase activity in prokaryotes d) the absence of a nucleus in prokaryotes

9 30205

When s subunit dissociates from an initiated RNA polymerase, a) it can bind a core enzyme to reform holoenzyme. b) it hydrolyzes ATP until rebound by core enzyme. c) it leaves behind an elongating species complexed with Rho factor. d) it remains bound to the promoter consensus sequence.

1 1847

The junction between the two helical portions of tRNA is stabilized by many non-standard base-base interactions between residues in the a) anticodon and CCA ends. b) helical stems of the D and Anticodon stems. c) loops of the Anticodon and Acceptor stem. d) loops of the TYC and D arms.

1 1542

On the ribosome, mRNA binds ____; the peptidyl transferase reaction occurs ____. a) to the large subunit; on the small subunit. b) to the small subunit; on the large subunit. c) between the subunits; on the large subunit. d) between the subunits; on the small subunit.

2 2852

According to the fluid mosaic model of cell membranes, which type of molecule spans the membrane from its inner to outer surface? a) cholesterol b) phospholipid c) protein d) triacylglycerol

PH.D Interview,

1 2834


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Un-Answered Questions { Bio Chemistry }

How are free energy changes related to enthalpy changes and entropy changes?

842


Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

918


why we use 96 well plate in ELISA plate reader?

1118


I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.

240


Which compound is involved in reducing levels of homocysteine in the blood?

1080






What are species grow in waitland?

1218


Why does the pH of the blood decrease in a person who has digested trematol?

1066


why we use 96 well plate in ELISA plate reader?

1104


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

914


All technical,written test questions

438


Where do hydrophobic and hydrophilic residues usually end up after protein folding?

897


what is QC and why is impotent in pathology lab

1527


How are free energy, equilibrium and spontaneity related to each other?

962


why ph is not more than 14?

236


What is zone electrophoresis?

928