you had 1,500 base pair pieces of random DNA and you
wanted to know how many of them had homology to known
genes, what would you do to determine that?

Answers were Sorted based on User's Feedback



you had 1,500 base pair pieces of random DNA and you wanted to know how many of them had homology ..

Answer / jomy peter thomas

just do blastn and count the number of sequences showing
more than equal to 30% similarity with the known sequences
in the databases.this wud give u th no. of homologous sequences.

Is This Answer Correct ?    2 Yes 0 No

you had 1,500 base pair pieces of random DNA and you wanted to know how many of them had homology ..

Answer / guest

use a BLASTX search against a known protein database - such
MCBI's NR database. I would then sort them to determine how
many were unique.

Is This Answer Correct ?    1 Yes 0 No

Post New Answer

More Bio Informatics Interview Questions

Are all members of transporter families confined to a particular organelle or to the plasma membrane, or are some family members found throughout cellular compartments in eukaryotes?

0 Answers  


How you caluculate sensitivity and selectivity of Blast?

2 Answers  


How to Count number of nucleotide substitutions ?

0 Answers  


What are the responsible factors for extinction of flora and fauna ?

3 Answers   CAT,


Describe the aim of microarray data analysis?

0 Answers  






If a match from a sequence database search is reported to have an E-value of 0.0, should it be considered highly insignificant or highly significant?

1 Answers  


? : BGLQ : : YDIN : VAFK

1 Answers   Wipro,


How would you value a biotech company as opposed to a consumer products company?

2 Answers   AgriGold,


Which of the following is not an essential condition for interference (a) The two interfering waves must be propagated in almost the same direction or the two interfering waves must intersect at a very small angle (b) The waves must have the same time period and wavelength (c) Amplitude of the two waves should be the same (d) The interfering beams of light must originate from the same source.

2 Answers   Wipro,


Ravi?s salary was reduced by 25%.Percentage increase to be effected to bring the salary to the original level is (a) 20% (b) 25% (c) 33 1/3% (d) 30%

3 Answers   Wipro,


what are databases used in bioinformatics?

1 Answers  


Does multidrug resistance (mdr) arise by activation of stable genes encoding drug efflux pumps or by mutations of genes encoding other types of transporters in bacterial pathogens?

0 Answers  


Categories