Which form of plasmid DNA moves quickest thro Agarose gels?
Answers were Sorted based on User's Feedback
Answer / ramesh singh kunjwal
Plasmid is present in the bacterial cell in the three
distinct forms.
1. Supercoiled
2. Nicked
3. Linear
Through the agarose gel the Supercoiled form move
Quickest.
Is This Answer Correct ? | 40 Yes | 4 No |
Answer / ambalika
In order, from slowest running to fastest running, they are relaxed or nicked circular (supercoling not yet accomplished [relaxed] or removed [nicked, a single stranded break]), linear (double stranded break), and supercoiled.
Is This Answer Correct ? | 10 Yes | 2 No |
Answer / sourav saha
There are 3 conformation observed on 1% agarose gel run of isolated Plasmid DNA, they are -- supercoiled circular DNA, nicked supercoiled circular DNA, and relaxed circular Dna. If the isolated plasmid DNA is not treated with Rnase enzyme then another band is observed in agarose gel known to be DNA masked with RNA. Read lehninger and stryer.
Is This Answer Correct ? | 6 Yes | 2 No |
Answer / sridhar
usually on the basis of mol weight the speed of DNA
depends..so the answer is single std DNA
Is This Answer Correct ? | 7 Yes | 10 No |
how much vol of sample u've 2 take to make 6mM solution.. if the Molr Wt is 164?
I am a final year student of bio_technology.I dont know how to make my profile?where is the opportunities for my studies?how is the interview flow?
How interferon is produced?
Sir i completed m.sc.biotech but for good career i want to do some diploma courses for good job . Will you please suggest me the coures
why the word trasfection is prefers for transfer of genetic material through non viral particles ineukaryote not for prokaryote?
Name some human products that are now synthesized by bacteria?
Which hemeprotein helps to store oxygen in muscle tissues?
Size of Normal Land and bhosada
What is isotopic tracer technique?
4 Answers ABC, KOL, Ranbaxy, Vimta Labs,
Give the comparisons of Rec-type (proteine) and Rec BCD Complex. What is the role of each?
1 Answers National University of Rwanda,
Agar is a polymer of which of the following? a. glucose b. galactose c. galactosamine d. fructose
I am doing my m.sc biotechnology.but i don't know about the interview questions of various company and how i have to face.can you please anyone send that placement papers?thank you