What reagents can be used to break disulfide bonds?
Answers were Sorted based on User's Feedback
Answer / raghavendra naidu
1)DTT- Di thio tritol
2)BME- Beta mercapto ethanol
Is This Answer Correct ? | 38 Yes | 0 No |
Answer / g.rajesh kumar
Mercaptoethanol breaks the disulphide bonds in proteins and
sds makes the protein to possess negative charge, so that
proteins can be separated based on their charge in sds-page
chromatography.
Is This Answer Correct ? | 23 Yes | 1 No |
Answer / narayana
Di thio thretol,Beta marcapto ethanol.
Some times urine also.
Is This Answer Correct ? | 2 Yes | 0 No |
Answer / g satheesh
sodium bisulfite in Base media or Zinc and acetic acid
Is This Answer Correct ? | 3 Yes | 2 No |
Answer / kesava.sat@gmail.com
SDS pocess the proteins to positive charge and b-mercato ethanol degrades the disulphide bonds. and urea also degrades the disulphide bonds
Is This Answer Correct ? | 1 Yes | 4 No |
When a forensics laboratory tests evidence collected at the scene of a crime using the "DNA Fingerprinting" technique, what are the technicians comparing?
What do you like LEAST & MOST about your current position?
What is the difference between pH and pKa?
what are the disadvantages of immobilized enzymes
What are the common features of active site?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
what are gangliosides?where they are found?
What are species grow in waitland?
A 3.00 * 10-6M solution in a 1.0 cm cuvette read 16 % T at 620nm. What were the absorbance and the molar absorbancy index of the solution?
what is Alzheimer's disease?what are its symptoms?
In water molecule the bond angle decreases from 109.28 to 104.5, why this does happens?
Give the chemical formula of Garnet?