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What types of changes can be used to effect fractional
precipitation of proteins?
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Answer / vipul panchal
various parameters r available.
1) pH- by using various pH change we can precipitate proteins having pI in the same range/
2)organic solvent- since each protein varies in their proportion of hydrophilic to hydrophobic we can make the use of increasing conc. of organic solvent thr by making the use of dielectric constant to isolate the proteins.
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Answer / vipul panchal
various parameters r available.
1) pH- by using various pH change we can precipitate proteins having pI in the same range/
2)organic solvent- since each protein varies in their proportion of hydrophilic to hydrophobic we can make the use of increasing conc. of organic solvent thr by making the use of dielectric constant to isolate the proteins.
3) Temperature- since some proteins are heat resistant this parameter can be use to isolate such protein.
4) salt conc.- by gradually increasing salt conc. we can fractionate the different proteins since depending upon their composition they differ in their solvation energy which can be change by changing ionic strength of solution.Process involves concept of salting out and salting in.
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Answer / rahul
salting out- add salts of higher ionic strength
decrease sulubility by adding organic solvents..
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