Why is it necessary to cleave proteins with at least two
different reagents during sequencing?



Why is it necessary to cleave proteins with at least two different reagents during sequencing?..

Answer / quido

generally the protein or polypeptide to be sequenced needs
to be cleaved into segments of size suitable or amenable to
the sequencing technique.

2 different reactions with two different cleaving agents is
used in order to allign the segments correctly once
sequencing is completed, with the help of overlapping
sequences, from the two digests.

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