How immunoglobulin fold looks like?
Why is not it correct to assert that dna self-replicates?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
How can proteins be denatured and renatured?
southern blot technique is related to--------- [fill the gaps]
What is the major advantage of Edman’s reagent?
what are the two types of methylmalonic acidemia(aciduria)?
what are 3 types of plasmalogens?
What is the difference between Phospholipids and phosphosphingosides?
which is more important, TCA or glycolysis??
What factors can change the pKa value of an amino acid residue, and in which direction?
how rotenone acts as an inhibitor of electron transport?
What amount of catalyst is consumed in the reaction it catalyzes?