How to establish relative response factor for hplc. Why it is required?
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In Agilent HPLC calibration using Empower 3 Software, What is the criteria for GPV Baseline starting point? i.e., Either it can start from -0.00/+0.00 or any range is there like -0.05 to +0.05 or ......etc?
what is regration method in method validation , how we can calculate it.
why we measure PH of a solution in PH only why not POH ?
1 Answers Stellence Pharm Science,
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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