Answer / harshad raj

In HPLC & GC :
The RT means, in which time the main principle peak of component is retain in minute called RT as retention time. and the RRT means: in which time the impurity peaks are retained before and after the main principle peak and that retention time of impurity peaks called RRT, ie. relative retention time RRT to the base of main peak RT.
The relation between RT & RRT is always the main peak RRT is 1.0 and the impurity peak RRT calculation is IMPURITY PEAK RT / MAIN PEAK RT.
For example: The main peak RT is 15 MIn, and the Impurity RT is 5 Min,
The RRT of Main Peak is 15/15 = 1
The RRT of Impurity peak is 5/15 = 0.333...

In Number of Theoretical Plates (N) = 16 (tr/w)2. where tr: retention time, and W: peak width. My Question is here in formula what is 16.Can u explain briefly? ---Veerabhadrarao.M

3 Answers   Micro Labs, Richter Themis, Sun Pharma,

---

why we prefer kbr for ftir than kcl?

5 Answers   Aurobindo,

---

When you get Moisture content by KF higher than by LOD, what does this indicate?

7 Answers  

---

How decided 10 ppm standard solution in Swab, rinse method.?

2 Answers  

---

what is mean by least count balance?

1 Answers  

---

What is linearity in hplc and how to calibration

1 Answers   Aurobindo,

---

same sample on two preapration gives differnt result during hplc analysis

3 Answers  

---

Why monograph of hplc coming upperside of drift but in IR monograph coming in drift side?

1 Answers  

---

What is normality

12 Answers   Cadila,

---

What is difference between assay by Hplc and uv

2 Answers   Aizant, Fredun Pharmaceuticals, Hetero, Marksans Pharma,

---

whate is difference between calibration and validation

11 Answers   Alkem Labs, Cipla, Ranbaxy, Torrent Pharma, Zydus Cadila,

---

What impact analysis required for out of calibration and how to proceed

2 Answers   Aventis,

---