Why it is OD 600nm for optical density investigation ?
- 2 Answers
- 20771 Views
- CDRI, Venus, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / shivesh
When measuring light scattering it is important to consider the wavelength of light used a bacterial culture. Microorganisms may contain numerous macromolecules that will absorb light, including DNA (254 nm), proteins (280 nm), cytochromes (400-500 nm), and possible cell pigments. When measuring bacteria by light scattering it is best to pick a wavelength where absorption is at a minimum and for most bacterial cultures wavelengths around 600 nm are a good choice. However, the exact wavelength chosen is species specific.
This wavelength is not absorbed by much of anything in bacteria so you measure light scattering by the microbes, which is proportional to cell density.
| Is This Answer Correct ? | 35 Yes | 2 No |
Answer / neha mumtaz
The OD600nm for optical density is considered as standard
because at this wavelength maximum absorption is observed.
| Is This Answer Correct ? | 28 Yes | 39 No |
Name four culture media used for Vibrio cholerae?
What is the HIV-1 Gag primer sequence ?
Explain the Esterase and lipase plate assay ?
who is genious of indian microbiologist?
6 Answers DRR, Reddy Labs, TATA,
Describe the symptoms ,transmission and treatment of cryptococcosis.
Which scientist provided the final disproof of spontaneous generation?
in autoclave sterilization time is 20mins,why?
Give an account of microbial polyphosphate?
What is meant by biotransformation and swertiamarin?
what is the use of MPN analysis?
what is a cytopathic effect?
Why does frame shift mutation (GC gase paired) occurs due to the pesticides in TA98 salmonella tester strain? What is its reason and the mode of action of the pesticides on the CG sequence of the DNA of TA98 strain
Micro Biology 
