What is the difference between Normal phase method &
Reverse method in HPLC ? Define application of both method.
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Answer / k l srinivas
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
| Is This Answer Correct ? | 57 Yes | 1 No |
Answer / maulik
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon
normal phase used for chiral compound and reverse phase used
for other
| Is This Answer Correct ? | 20 Yes | 2 No |
Answer / srikanth
above answer is perfect and also,,,....
Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.
Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure
| Is This Answer Correct ? | 7 Yes | 0 No |
Answer / ram krishna
Normal phase _Column is polar and more is non polar sudh as IPA hexane
Reversed phase_Column is non polar and more is polar such as water and acetonitrile mp
| Is This Answer Correct ? | 2 Yes | 0 No |
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Organic Chemistry 
