what r the reasons to get peak telling?
Answers were Sorted based on User's Feedback
Answer / ravi kumar
due to the presence of residual silanols on the reverse
phase column which interacts with acids or bases (mostly
bases)cause them to tail
Is This Answer Correct ? | 19 Yes | 1 No |
Answer / nageswar rao
the following parametrs are reasons
1.silinol interaction of basic amines
2.adsorption of acidic compounds
3.mass overloaded
4.injecting sample in worng solvent significantly stroger
than mobile phase
Is This Answer Correct ? | 15 Yes | 1 No |
Answer / nagesh.v@sai advantium.com
Due to column efficiency Decreaced
Is This Answer Correct ? | 11 Yes | 2 No |
Answer / subash
The following reasons are causes of peak tailing
1.Injecting the sample in a solvent significantly stroger
than the mobile phase
2.Interaction of stationary phase silanols with basic
analyte
3.Adsorption of acidic compounds on the stationary phase
4.Sample mass overloaded
5.A void in the column packing bed
Is This Answer Correct ? | 5 Yes | 0 No |
Answer / t.venkata reddy
below reasons causes peak tailing
1)Analytical column contaminated
2)Mobile phase contamination/deterioation
3)unswept dead volume
4)basic substance-silanol interation.
Is This Answer Correct ? | 2 Yes | 0 No |
Answer / palabindela
column over loaded or high injection volume
Is This Answer Correct ? | 0 Yes | 2 No |
what you do when compound having dwel wavelength?
In the test for control of absorbance in UV calibration why do we use only potassium dichromate and what is purpose of taking a specified amount of 57.0-63.0mg?
Why dissolution apparatus having 8 jars ?
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
Explain in detail what are the procedure and calculations involved for accuracy (%Recovery)in method validation?
Near infrared Principle
Why will a solution of aspirin without acid or basic hydrolysis give yellow colour with a solution of Ferric chloride?
what type of compounds do we see lod @ wat type compounds do we see m.c
why we use holmiumperchlorate in calibrating uv
4 Answers Aurobindo, DRL, MSN Pharma,
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
why we are use metthanol variously in kfr?why don't use other solutions?
1 % solution is equal to how much ppm