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what r the reasons to get peak telling?
- 6 Answers
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Answer / ravi kumar
due to the presence of residual silanols on the reverse
phase column which interacts with acids or bases (mostly
bases)cause them to tail
| Is This Answer Correct ? | 19 Yes | 1 No |
Answer / nageswar rao
the following parametrs are reasons
1.silinol interaction of basic amines
2.adsorption of acidic compounds
3.mass overloaded
4.injecting sample in worng solvent significantly stroger
than mobile phase
| Is This Answer Correct ? | 15 Yes | 1 No |
Answer / nagesh.v@sai advantium.com
Due to column efficiency Decreaced
| Is This Answer Correct ? | 11 Yes | 2 No |
Answer / subash
The following reasons are causes of peak tailing
1.Injecting the sample in a solvent significantly stroger
than the mobile phase
2.Interaction of stationary phase silanols with basic
analyte
3.Adsorption of acidic compounds on the stationary phase
4.Sample mass overloaded
5.A void in the column packing bed
| Is This Answer Correct ? | 5 Yes | 0 No |
Answer / t.venkata reddy
below reasons causes peak tailing
1)Analytical column contaminated
2)Mobile phase contamination/deterioation
3)unswept dead volume
4)basic substance-silanol interation.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / palabindela
column over loaded or high injection volume
| Is This Answer Correct ? | 0 Yes | 2 No |
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