What is 'polymerase chain reaction'?

Answers were Sorted based on User's Feedback



What is 'polymerase chain reaction'?..

Answer / sunilreddy.vancha

A technique for amplifying DNA sequences in vitro by
separating the DNA into two strands and incubating it with
oligonucleotide primers and DNA polymerase.

Is This Answer Correct ?    4 Yes 1 No

What is 'polymerase chain reaction'?..

Answer / d.jeeva jothi

The Polymerase chain reaction (PCR) is an effective
technique that helps produce multiple copies of a target DNA
sequence.

It amplifies DNA that are either too small, or too degraded.
Thus it is possible to analyse DNA got in minute quantities
in places like a drop of blood at a crime scene or a cell
from an extinct dinosaur.

Thermal cycler is an instrument which is used to run the PCR
experiments. PCR was discovered by Kary Mullis in 1984.

Is This Answer Correct ?    1 Yes 0 No

What is 'polymerase chain reaction'?..

Answer / atul gaur

+with the help of different temperatures
1> denaturation 94 c
2> annealing 54 c
3> synthesis 74 c

Is This Answer Correct ?    1 Yes 1 No

Post New Answer

More Bio Chemistry Interview Questions

What is betapropiolactone?

3 Answers   Aurobindo, Himadri,


When it functions as a "second messenger", cAMP a) acts outside the cell to influence cellular processes. b) acts "second in importance" to AMP. c) activates all cytosolic protein kinases. d) activates the cAMP-dependent protein kinase.

0 Answers  


The junction between the two helical portions of tRNA is stabilized by many non-standard base-base interactions between residues in the a) anticodon and CCA ends. b) helical stems of the D and Anticodon stems. c) loops of the Anticodon and Acceptor stem. d) loops of the TYC and D arms.

1 Answers  


What does thermodynamics helps in predicting?

0 Answers  


Which pyrimidine base contains an amino group at carbon 4?

1 Answers  






Describe the classification of all amino acids .

4 Answers  


What kinds of proteins are involved in facilitating protein folding?

1 Answers  


Explain why nitrogen cannot form penta halides?

0 Answers  


How can we calculate the number of possible optical isomers for a given compound?

0 Answers  


Autoanalyser is used for[tick your right choice} 1.Analysing heart defect 2.Imaging various internal body parts 3.Estimating requirement of surgical removal or correction 4.Estimating quantity of various biochemicals in body fluids

4 Answers  


Which is the least abundant Alkaline earth element?

0 Answers  


Is there any metabolic pathway for water? If not is it possible to provide one?

3 Answers  


Categories