In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
Atul,
1 6533In capadity factor formula is K'= (RT of main compound/RT of first eluted peak)-1 in this formula what is 1
1 6257hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 3483Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 8103some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
2099Post New Analytical Chemistry Questions
Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?
iam usig ph buffers merk. manually how to prepare ?
what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?
How you develop a method in HPLC?
What is split ratio in Gc? Splitless? how requirr to select?
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
why digestion require in icpms?
How would you decide dissolution medium for NCE compound of class I drug
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
What is shaking level in GC?
in which situation ion pair require to use?
what is the extinction coefficient for Indomethacin or at 319nm wave length.
If combination product how require to identify which imp is of which api?
[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?
In the isomers,enantiomers give the only physical properties present such that no chemical properties present.
