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Why is it usually necessary to cleave proteins into smaller
fragments for sequencing?
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Answer / guest
it is not nessesary, but
there is always errors when removins an amino acid
if the protein is long the will be an incresing noise in
the estimated sequence
(this is called error porpagation)
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Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?
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