Which enzymes in cows allow them to digest cellulose?

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how do u differentiate pseudoglobulins and euglobulins on the basis of solubility?

1 Answers  

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how bpg acts as a haemoglobin regulator?

2 Answers  

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What types of changes can be used to effect fractional precipitation of proteins?

3 Answers  

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Define polar covalent bond?

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how the keratosulphate differs from other mucopolysaccharides?

1 Answers   Aurobindo,

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What is the difference between and aldose and a ketose? Give an example of each?

2 Answers  

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What is nucleophilic addition reaction?

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what is RFLP?

1 Answers  

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?

1 Answers   Pace Pharmaceuticals,

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The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

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