How to detect receptor on the cell surface?
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Answer / Mahesh Pratap Singh Sikarwar
Receptors on the cell surface can be detected using several methods, including flow cytometry, immunocytostaining, and biotinylation. In flow cytometry, fluorescently labeled antibodies against the receptor of interest are used to stain the cells, followed by analysis on a flow cytometer. For immunocytostaining, cells are fixed, permeabilized, and incubated with primary antibodies against the receptor, followed by secondary antibody conjugated to a fluorescent dye. Biotinylation involves labeling cell surface proteins with biotinylated probes, which can then be isolated using streptavidin-coated beads and analyzed.
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