What is the pore size of nitro cellulose memberane used in soythern blotting technique?

1 Answers  

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What causes the elasticity of corneal layer of skin?

0 Answers  

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what is distribution ratio in chromatography technique?

2 Answers   Nutrition Research Laboratories,

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If the enthalpy change for a reaction is zero, DG? is equal to: a) DH? b) lnKeq c) TDS? d) -TDS?

1 Answers  

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what are anaplerotic pathways?

6 Answers   Reddy Labs,

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The nucleophile in the first stage of the serine protease mechanism is a) H2O b) the carboxyl of Asp 102 c) the hydroxyl of Ser 195 d) the imidazole of His 57

0 Answers  

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What is meant by non-conjugated double bond system?

1 Answers  

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The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

0 Answers  

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how will u identify which one of the given 2 test tube contains protein solution or which one contains DNA solution without using any instrument?

5 Answers  

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What does law of mass action states?

0 Answers  

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How immunoglobulin fold looks like?

1 Answers  

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

0 Answers  

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