Explain steps involved in transcription?
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Answer / Gauri Shankar Tiwari
Transcription is the process of creating an mRNA copy (messenger RNA) from a DNA template. Here are the main steps involved:
1. Initiation:
- The enzyme RNA polymerase binds to the promoter region of the gene and forms an initiation complex.
- The enzyme unwinds the double-stranded DNA around the start site, creating a single-stranded template for mRNA synthesis.
2. Elongation:
- RNA nucleotides are added sequentially to the 3' end of the growing mRNA strand according to the base pairing rules (A pairs with U or T, C pairs with G).
- This process continues until a termination signal is reached on the DNA template.
3. Termination:
- RNA polymerase encounters a terminator sequence on the DNA template, which triggers the release of the newly synthesized mRNA molecule.
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