How to get rid of contamination in stable transfected cells?
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Answer / Harendra Kumar Yadav
Contamination in stable transfected cells can be addressed by several methods. These include:n- Antibiotic selection: Using antibiotics that kill the contaminating organisms while sparing the transfected cells.n- Cloning of individual cells: Isolating single transfected cells and expanding them separately to ensure purity.n- Flow cytometry sorting: Separating transfected cells based on specific markers using flow cytometry.
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