How C2C12 cells model for differentiation?
- 1 Answers
- 2461 Views
- I also Faced
- E-Mail Answers
Answer / Chandan Kumar Rawat
C2C12 cells, a mouse myoblast cell line, are commonly used to study skeletal muscle development and differentiation. To induce differentiation, C2C12 cells are typically cultured in high serum (usually 10-20% fetal bovine serum) conditions for several days until they reach confluence. At this point, the cells are switched to low serum (typically 2%) or serum-free media containing various growth factors such as insulin and dexamethasone, which promote muscle differentiation. Over a period of several days, C2C12 cells will begin to fuse together and express muscle-specific markers like myosin heavy chain (MHC), indicating successful differentiation.
| Is This Answer Correct ? | 0 Yes | 0 No |
What is proteosome inhibitor?
Describe about Fusing spleen cells with myeloma cells using PEG ?
what substances is the nucleolus made? Is there a membrane around the nucleolus?
what are ATP lowering agents?
Describe the technique for Centromere protein staining ?
why artificial blood can not be manufactured ?
Where in the cell can ribosomes be found?
Can mechanical freezers be used for cryogenic storage of cultured cells?
What is the energy source used in active transport through biological membranes?
what are the disadvantages of soft agar assay?
Explain the synchronization of cancer cells ?
what is the role of non-radioactive ATP analog for kinase assay?
Micro Biology 
