Interested to Buy Any Domain ? << Click Here >> for more details...
How to extract the Proteins from gram-positive bacteria In
particular Staphylococci ?
- 1 Answers
- 4630 Views
- I also Faced
- E-Mail Answers
Answer / sunil.vancha
Staphylococcal cell protein and urease can be solubilized
after growth in Todd-Hewitt broth supplemented with 0.5%
yeast extract by extraction for 18–24 h in phosphate
buffer, pH 7.0. In general 20% (but up to 100%) of the
urease present in the original cells could be solubilized.
Less protein was solubilized. Species examined included
coagulase-negative staphylococci, Staphylococcus
intermedius and Staph. aureus. Extracts of Staph,
epidermidis prepared by this procedure gave electrophoretic
urease and protein patterns similar to those prepared by
sonication. The procedure was simple and minimized handling
of the cells.
| Is This Answer Correct ? | 0 Yes | 0 No |
what is meant by DNA shuffling?
What is the Problem with a chromogenic Indolyl-L-arabinoside in E .coli?
Explain the symptoms of trichinosis?
What are Hybridomas?
2 Answers Biological.e.limited,
how can i calculate the bacterial endotoxin limit of ringer's acetate intravenous infusion solution?
0 Answers Fipco Pharma & Pharmaceutical Corporation,
Which scientist provided the final disproof of spontaneous generation?
Which are the alternatives used in gram stain?
What is the ph range of most bacteria and most fungai.
Contrast the streak plate isolation and pour-plate isolation methods for making population measurements?
Who provided the experimental evidence that led to the gene- for-gene theory?
What is the media used for MPN analysis?
How to keep stock of fungi for long term ?
Micro Biology 
