Explain about the RNA isolation from bacteria?
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Answer / Sachin Pandey
RNA isolation from bacteria involves several steps to ensure that the extracted RNA is pure and intact. First, cells are harvested by centrifugation and washed with sterile water or buffer. The cells are then disrupted using mechanical methods such as bead beating or sonication. Next, cell debris is removed by centrifugation, and RNA is purified using phenol-chloroform extraction or commercial kits that utilize column chromatography. Finally, the RNA is precipitated with ethanol and resuspended in RNase-free water.
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