Interested to Buy Any Domain ? << Click Here >> for more details...
How can one separate prokaryotic m-RNA
- 4 Answers
- 8432 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / kush
Separate the nucleoid (portion containing nucleic acids) of
the prokaryote by cell lysis and differential
centrifugation. After that, treat the extract with DNAse to
remove any trace of DNA along with the RNA. The RNA thus
obtained is mRNA.
| Is This Answer Correct ? | 1 Yes | 1 No |
Answer / abbineni durgaprasad
first we have to isolate complet RNA THEN apply that on
oligo dT Cellulose column then you can get your m-RNA
| Is This Answer Correct ? | 3 Yes | 5 No |
Lyse the cell first then centrifuge in cesium chloride
gradient then separate out Genetic material and then use
DNAase enzyme to lyse DNA then extract rna by centrifugr or
gel electrophoresis method
| Is This Answer Correct ? | 0 Yes | 2 No |
Answer / sharun
prokaryotes do not produce mRNA... this is produced in the
riobosomes of Eukaryotes... prokaryotes produce rRNA which
can be separated by lysing the Shine-Dalgarno sequence with
BBNT and then applying a
dideoxyoligonucleotidephosphohydroxylase to the upstream
portion of your okazaki fragments
| Is This Answer Correct ? | 0 Yes | 5 No |
What is novel feed?
sir i completed my B.E Bio technology please suggest me which is the better course either M.TECH or any diploma course & i intrested to do clinical research,please suggest me any one
tell me about your career aspiration
How DNA is being Transplanted?
Suppose that an investigator wanted to use DNA fingerprinting to associate a blood sample with a criminal. What materials would be necessary?
What is necessary for commercial production of a biotech plant?
What type of vectors are used in ex vivo gene therapy?
Which univ is better for biotechnology- wayne state univ or georgetown univ
What kinds of metrics would you gauge to determine the financial, strategic and operational health of a prospective alliance partner?
What do you mean by bio ethics?
What is the difference between traditional breeding and recombinant DNA techniques?
The absorbance of protein at 280 nm is due to which amino acid?
Micro Biology 
