Interested to Buy Any Domain ? << Click Here >> for more details...
you have a mixture of dNTPs(deoxynucleotide triphosphates)in a
sample.how can you separate these all?
- 3 Answers
- 6869 Views
- IISc, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / jvishuu007
this may be done by the use of affinity chromatography.take a matrix lablled with dATP in a column.run the mixture from the top of column.only dTTP will bind to the column, rest are eluted.this dTTP then separated from matrix by increasing temperature..use this concept to separate other dNTPs.
| Is This Answer Correct ? | 17 Yes | 4 No |
Answer / vipul panchal
I think we can use thin layer chromatography.
just run standards that is dATP,dGTp, dTTp, dCTP, dUTP.
compare this standard gel with yr test gel.
| Is This Answer Correct ? | 0 Yes | 2 No |
Answer / tanu talwar
by modifide the bases and run them on the cellulose gel.
| Is This Answer Correct ? | 0 Yes | 3 No |
How do peptides react with cyanogen bromide?
How are free energy, equilibrium and spontaneity related to each other?
Which type of chemical bond maintains the pairing of each chain in the dna molecule?
What functional group is hydrolyzed in the hydrolysis of ATP to ADP and Pi?
What are the types of chromatography?
Because of dna replication, two dna molecules come to existence. Why is not it correct to assert that two “new” dna molecules are created? What is the name given to the process concerning that fact?
how rotenone acts as an inhibitor of electron transport?
Covalent bond is directional therefore which covalent property is shown?
What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
What determines whether a B-cell will respond to an antigen?
what are anaplerotic pathways?
how haemoglobin acts as an allosteric protein?
Micro Biology 
