you have a mixture of dNTPs(deoxynucleotide triphosphates)in a
sample.how can you separate these all?
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Answer / jvishuu007
this may be done by the use of affinity chromatography.take a matrix lablled with dATP in a column.run the mixture from the top of column.only dTTP will bind to the column, rest are eluted.this dTTP then separated from matrix by increasing temperature..use this concept to separate other dNTPs.
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Answer / vipul panchal
I think we can use thin layer chromatography.
just run standards that is dATP,dGTp, dTTp, dCTP, dUTP.
compare this standard gel with yr test gel.
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Answer / tanu talwar
by modifide the bases and run them on the cellulose gel.
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