How does the UV absorption of specific amino acids aid biochemists?1 1781
How does isoelectric focusing work?1 3360
What are some commonly used biochemical buffers?3 2048
What role does the primary structure play in determining the folding pattern of a protein?1 2142
How does the stability of peptides compare with that of amino acids?2 1752
What is meant by “cooperative folding” of proteins?1 2150
Why does ion-exchange chromatography generally use a salt gradient or a pH gradient?1 4611
What factors cause salting in and salting out of macromolecules?1 2072
Compare and contrast feedback inhibition and enzyme repression?
How are free energy changes related to LeChatelier's Principle?
"Tell me about a job that you found particularly satisfying. What did you like about it?
The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.
How much empty space is found in globular proteins?
what are the steps involved in the calibration of HPLC
How are reaction rates dependent upon free energy changes?
Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O
What type of column is generally used to separate amino acids from each other?
Which compound is involved in reducing levels of homocysteine in the blood?
What are hydrophobicity scales, and how are they used?
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
Describe the structure of a peptide giving the sequence.
Where do hydrophobic and hydrophilic residues usually end up after protein folding?
What is the structural formula of molybdenum blue?