Bio Chemistry Interview Questions
Questions Answers Views Company eMail

How are free energy changes related to LeChatelier's Principle?

864

How does the UV absorption of specific amino acids aid biochemists?

1 1781

How does isoelectric focusing work?

1 3360




What are some commonly used biochemical buffers?

3 2048

Describe the structure of a peptide giving the sequence.

768

How do peptides react with cyanogen bromide?

860

How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?

995

What role does the primary structure play in determining the folding pattern of a protein?

1 2142




How is the actual free energy change for a process related to the standard free energy change?

1566

What factors can change the pKa value of an amino acid residue, and in which direction?

1396

How does the stability of peptides compare with that of amino acids?

2 1752

How does hplc differ from normal column chromatography, and what are its advantages?

1027

What is meant by “cooperative folding” of proteins?

1 2150

Why does ion-exchange chromatography generally use a salt gradient or a pH gradient?

1 4611

What factors cause salting in and salting out of macromolecules?

1 2072


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Un-Answered Questions { Bio Chemistry }

Compare and contrast feedback inhibition and enzyme repression?

8314


How are free energy changes related to LeChatelier's Principle?

864


"Tell me about a job that you found particularly satisfying. What did you like about it?

1972


The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

2024


How much empty space is found in globular proteins?

952






what are the steps involved in the calibration of HPLC

1516


How are reaction rates dependent upon free energy changes?

1111


Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O

1520


What type of column is generally used to separate amino acids from each other?

1024


Which compound is involved in reducing levels of homocysteine in the blood?

1199


What are hydrophobicity scales, and how are they used?

828


Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

2032


Describe the structure of a peptide giving the sequence.

768


Where do hydrophobic and hydrophilic residues usually end up after protein folding?

970


What is the structural formula of molybdenum blue?

2410