Bio Chemistry Interview Questions
Questions Answers Views Company eMail

How are free energy changes related to LeChatelier's Principle?

794

How does the UV absorption of specific amino acids aid biochemists?

1 1663

How does isoelectric focusing work?

1 3168




What are some commonly used biochemical buffers?

3 1918

Describe the structure of a peptide giving the sequence.

714

How do peptides react with cyanogen bromide?

792

How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?

837

What role does the primary structure play in determining the folding pattern of a protein?

1 2028




How is the actual free energy change for a process related to the standard free energy change?

1480

What factors can change the pKa value of an amino acid residue, and in which direction?

1312

How does the stability of peptides compare with that of amino acids?

2 1624

How does hplc differ from normal column chromatography, and what are its advantages?

935

What is meant by “cooperative folding” of proteins?

1 2043

Why does ion-exchange chromatography generally use a salt gradient or a pH gradient?

1 4356

What factors cause salting in and salting out of macromolecules?

1 1963


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Un-Answered Questions { Bio Chemistry }

Why does physical exertion increase symptoms of poisoning by trematol?

1509


Glycolysis is A) C6H12O6 --> 2C2H5OH + 2CO2 B) C6H12O6 --> 2CH3H4O3 + 4H c) C3H4O3 + NAD --> C2H5OH + CO2 NAD D) C6H12O6 + 6H20 --> 6CO2 + 6H2O

1431


What factors can change the pKa value of an amino acid residue, and in which direction?

1312


Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

918


Why does the pH of the blood decrease in a person who has digested trematol?

1066






I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.

241


How are free energy changes related to LeChatelier's Principle?

794


why we use 96 well plate in ELISA plate reader?

1118


which fat soluble vitamin has co-enzymic function?

777


Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.

1963


What type of column is generally used to separate amino acids from each other?

960


Describe the environment where you do your best work in an organization?

953


Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?

963


why we use 96 well plate in ELISA plate reader?

1104


How are coupled reactions used in biochemistry?

1121