If you isolate mitochondria and place them in buffer with a low pH they begin to manufacture ATP. Why?
Explain why in anaerobic cells the ratio of pyruvate/ lactate is much less than 1 while under aerobic conditions the ratio of pyruvate/ lactate is much greater than 1?1 4585
Which cellular compartment becomes acidic during mitochondrial electron transport?2 3867
what is the primary purpose of fermentation in the absence of oxygen?1 4418
Name the terminal electron acceptor during mitochondrial respiration?1 5791
During a heart attack, blood flowing to the heart muscle is interrupted by blockage of a coronary artery. How would you expect the metabolism in the heart to change?1659
Reduction of which intermediate in glycolysis leads directly to a compound involved in the biosynthesis of fats?1 1754
Which organ and subcellular site are most important for fatty acid biosynthesis?1 1580
When a forensics laboratory tests evidence collected at the scene of a crime using the "DNA Fingerprinting" technique, what are the technicians comparing?1 1783
What is the difference between and aldose and a ketose? Give an example of each?2 13117
What are carbohydrates used for in living systems?3 7200
what is a Reducing sugar?3 2826
Where do hydrophobic and hydrophilic residues usually end up after protein folding?
why we use 96 well plate in ELISA plate reader?
What factors are involved in determining the electrophoretic mobility of a molecule?
does glucagon secretion induce sleep in teenagers?why?
"Tell me about a job that you found particularly satisfying. What did you like about it?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
Calculate the molar extinction coefficient of a solution containing 5 *10-4 g litre-1 of a biomolecule, molecular weight 275 g mol-1, and absorbance 0.75 in a 1.2 cm cuvette.
How are free energy changes related to enthalpy changes and entropy changes?
How are free energy changes related to LeChatelier's Principle?
what are the steps involved in the calibration of HPLC
Which organ and subcellular site are most important for fatty acid biosynthesis?
I understand that urine can act as a reagent to break disulfide bonds? I am particularly interested in bonds between cysteines. and or any other amino acids.
DNA binding by proteins with the helix-turn-helix (HTH) motif does not involve a) altered stacking of the DNA at the center of symmetry. b) hydrogen bonds, salt bridges, and van der Waals contacts. c) interactions with base pairs in the major groove of DNA. d) interactions with the sugar-phosphate backbone of DNA.
Why are free cysteines treated with iodoacetate prior to protein sequencing?
can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?