How to caluculate the RRF and correction factor in hplc
- 1 Answers
- 12778 Views
- Alkem Labs, Aurobindo, Rakshit Drugs, SDLPL, I also Faced
- E-Mail Answers
Answer / dr. harshvardhan joshi
RRF is nothing but relative retention factor. As the name suggests it is relative to something.
To calculate RRF, its first important to calculate RF (Response factor)
Reponse factor = Peak area of analyte/concentration of analyte
In order to find out concentration of unknown analyte, or when analytes response is very low with respect to the main analyte RRF calculation can be used. If there are two analytes, (X) and (Y) and if concentration of one is unknown. Then,
RRF = RF of Y/RF of X (RF values from above calculation)
This type of calculation is beneficial when we are using one of the analyte as internal standard
| Is This Answer Correct ? | 8 Yes | 4 No |
What is the defination of Bulk density?
How you start the method development of unknown compound.
Is sonication essential in solubility testing of a drug substance?
In gas chromatographic analysis sometimes we use headspace, why it is required instead of manually injection?
in dissolution if one bowl got 70 percent 2nd bowl got 80 percent and 3rd bowl got 90 percent then how proceed?
IF WE INCREASE FLOW RATE RT DECRESE.WHY
how to calculate corelation coefficient in G.C Calibration? what is the calculation part?
What is definition of RRT and what is the difference between RT and RRT in HPLC
why hexane is used for FID calibration in GC
Is it necessary to validate both assay and related substances for usp official methods for products.
what is peak purity ??
what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment
Organic Chemistry 
