From peak purity spectrum, where or at which point peak
purity angle & purity threshold is checked?
Answer / nikil kumar
At that point where Maxima and response obtained for main component and all known impurities at higher level to LOQ level
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give tha clarity about peak purity concept
3 Answers Aurobindo, Reliance Life Sciences,
In UV we measure absorbance , how it is happend ? explain me
In HPLC Chromatographic separation Resolution equation is Rs = 1.18 (tR2 –tR1)/wh1+Wh2, Where, tR2 and tR1 is run time of eluent ratios of the distances from the point of application to the centers of the spots and the distance travelled by the solvent front from the point of application (retardation factor), and Wh1and Wh2 , is peak width of at half height. What is 1.18 ?